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-trifluoroacetyl substituted substrate is hydrolyzed 26 periods faster compared to normal substrate though the N
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It could be argued, even so, that if NagA ended up minimal by merchandise release from the Energetic web-site, then the dissociation of trifluoroacetate could be inherently quicker than the release of acetate. This unique circumstance was resolved with the utilization of modifications in solvent viscosity to systematically alter the speed constants for that Affiliation and dissociation of products and solutions and substrates with NagA (19, twenty).
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